LC-MS/MS Data Analysis
2 Data Analysis
Install the Matlab Tool Peak Viewer. The software will pick peaks automatically based on highest correlation in the 12C (black) and 13C channel (orange). These are the black and orange dots. The ration between the 12C and 13C peak heights is the final read out (if you dont work with absolute concetrations).
LC-MS data analysis needs some experience. It varies between runs and with sample matrix (especially salts have a big effect).
The software does not pick every peak right. Here are the important metabolites that you must manually correct. Do this by clicking the "All Samples" radio-button, then use the slider to position the peak.
1 For targeted Metabolomcis we use LC-MS/MS. Detailed Infos are in the Method Paper: